OFF-THE-SHELF PRODUCTS
MISSION(R) TRC lentiviral shRNA Collection
The RNAi Consortium or TRC at the Broad Institute designed and created the shRNA library to enable the use of RNA interference (RNAi) technology to determine the function of human and mouse annotated genes. The short hairpin RNA (shRNA) constructs were designed to include a 21 base pair sense and antisense stem and 6 base pair loop cloned into lentiviral pLKO-puro vectors. The collection was developed in 2 phases, thus the sublibraries are also designated as TRC 1/1.5 (pLKO.1) and TRC 2 (pLKO.5).
Our facility houses the entire TRC collection: Individual clones, Pathway-specific Panels and Genome-wide Libraries.
Highlights:
1) Already cloned-in lentiviral vectors
2) U6-driven shRNA, puromycin resistance to select transduced cells
3) Available as bacterial glycerol stocks, plasmid DNA and lentiviral suspensions
4) Quick turn-around time
More Information: The Broad Institute, Millipore-Sigma
Instructions to look up shRNA clones
CCSB-Broad Lentiviral Expression Library
The Open Reading Frame (ORF) collection was developed at the Dana Farber Cancer Institute and the Broad Institute to enable the expression of ~15000 human ORFs. The ORF constructs were designed to express human ORF without the stop codons and a C-terminal V5-tag in a lentiviral PXL304 vector backbone.
Our facility houses the complete CCSB-Boad Lentiviral Expression Library: Individual clones
Highlights:
1) Already cloned-in lentiviral vector
2) CMV-driven ORF, C-terminal V5 tag, blasticidin resistance to select transduced cells
3) Available as bacterial glycerol stock, plasmid DNA and lentiviral suspension
4) Quick turn-around time
More Information: Dharmacon Horizon Discovery
Instructions to look up clones
Sanger Arrayed Whole Genome Lentiviral CRISPR Library (Human)
The Wellcome Sanger Institute and Millipore Sigma joined forces to create the first ever arrayed CRISPR knock out library for human and mouse genomes. The library was developed as a 2-plasmid system, where one plasmid is used to express CRISPR-associated endonuclease (Cas9) and a second plasmid is used to express guide RNA (gRNA).
Highlights:
1) Already cloned-in lentiviral vector
2) U6-driven gRNA; two gRNA per gene, puromycin resistance to select transduced cells, BFP selection for tranfected cells
3) Variety of Cas9 vectors to fit experimental need
4) Quick turn-around time
More Information: Millipore-Sigma
Instructions to look up clones
Cas9 options